Treponemal antibody-absorbent enzyme immunoassay for syphilis
نویسندگان
چکیده
منابع مشابه
Treponemal antibody-absorbent enzyme immunoassay for syphilis.
An enzyme immunoassay for the diagnosis of syphilis (ELISA-SY) was developed with solid-phase extracts of Treponema pallidum, specimen diluent containing Reiter treponeme absorbent, and three 30-min incubations. The ELISA-SY results were determined in comparison with a standardized positive control and reported as a percentage of strong positive control. In tests with 1,005 serum samples from a...
متن کاملClinical comparison of the Treponema pallidum CAPTIA syphilis-G enzyme immunoassay with the fluorescent treponemal antibody absorption immunoglobulin G assay for syphilis testing.
Recently, a treponema-specific immunoglobulin G (IgG) enzyme immunoassay (EIA), the CAPTIA Syphilis-G (Trinity Biotech, Jamestown, N.Y.), has become available as a diagnostic test for syphilis. A total of 89 stored sera previously tested by the fluorescent treponemal antibody absorption (FTA-ABS) IgG assay were evaluated by the CAPTIA EIA. The FTA-ABS IgG procedure was performed by technologist...
متن کاملComparative evaluation of three different treponemal enzyme immunoassays for syphilis.
BACKGROUND To reduce human errors and subjective interpretation, automation is currently a trend. However, replacing any tests with automation must first be validated. OBJECTIVE Evaluate the EIA tests performance characteristics of three commercially available enzyme immunoassays; Enzygnost Syphilis (Dade Behring Ltd), Syphilis EIA 480 (Newmarket Laboratory Ltd) and ICE* Syphilis (Abbott Mure...
متن کاملEvaluation of the automated fluorescent treponemal antibody test for syphilis.
The Automated Fluorescent Treponemal Antibody (AFTA) procedure developed by the Space Division of Aerojet General Corporation (El Monte, California) was tested in this laboratory as part of a field evaluation sponsored by the Venereal Disease Research Laboratory of the National Communicable Disease Center, Atlanta, Georgia (Stout, Lewis, Duncan, Hunter, and Lantz, 1968). The reactivity of the a...
متن کاملEnzyme immunoassay for anti-treponemal IgG: screening or confirmatory test?
AIMS To review the performance of the Venereal Diseases Research Laboratory (VDRL) test and the Treponema pallidum haemagglutination assay (TPHA) as a combined screen for syphilis to provide a baseline for assessing screening by anti-treponemal IGG EIA. METHODS Between 1980 and 1987 all serum samples were screened by both VDRL and TPHA tests. The FTA-ABS test was also used in suspected early ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1986
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.23.5.876-880.1986